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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Horm Behav. 2018 Nov 8;111:60–69. doi: 10.1016/j.yhbeh.2018.10.011

Figure 1.

Figure 1

Construction of sgRNAs targeting the prairie vole oxytocin receptor gene (Oxtr). (A) The target regions of each sgRNA in Oxtr. All sgRNAs were targeted against the 5’ region of the coding sequence (CDS). UTR = untranslated region. (B) Evaluation of cutting efficiencies by SSA assay in HEK293T cells (n = 3). The greater the number of EGFP positive cells, the greater the cutting efficiency, n.s = not significant. * = p<0.05. Data are presented as mean +/− Standard Error of the Mean (SEM). Scale bar = 150 μm.