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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Horm Behav. 2018 Nov 8;111:60–69. doi: 10.1016/j.yhbeh.2018.10.011

Figure 2.

Figure 2

Generation of Oxtr gene modified prairie voles by CRISPR/Cas. (A) Picture of vl, v2 and v3 mutant voles demonstrating their healthy appearance. (B) T7E1 assay for detection of indels induced by CRISPR/Cas. The presence of multiple bands is indicative of the introduction of indels.(C) Sequences of each mutant vole. Note the most voles had indels in both alleles of the Oxtr. Four mutant alleles in the v3 vole indicated that CRISPR might work at the 2-cell stage. Red letters = protospacer sequence; green letters = PAM; blue letters = insertion; magenta letters = conversion; hyphen = deletion.