KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Biological Samples | ||
| Preparation of HO shell | (Sutter et al., 2017) | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Nonidet P40 substitute | Sigma-Aldrich | Cat# 11332473001 |
| Deposited Data | ||
| Coordinates HO shell main population | this paper | PDB ID 6MZX |
| Map HO shell main population | this paper | EMD-9309 |
| Coordinates HO shell compacted | this paper | PDB ID 6MZY |
| Map HO shell compacted | this paper | EMD-9310 |
| Coordinates for subvolume with BMC-TS | this paper | PDB ID 6N06 |
| Map for subvolume with BMC-TS | this paper | EMD-9311 |
| Coordinates BMC-TD (main population, closed) | this paper | PDB ID 6MZU |
| Map BMC-TD (main population, closed) | this paper | EMD-9307 |
| Coordinates BMC-TD (main population, widened) | this paper | PDB ID 6MZV |
| Map BMC-TD (main population, widened) | this paper | EMD-9308 |
| Coordinates BMC-TD (compacted population, open) | this paper | PDB ID 6N07 |
| Map BMC-TD (compacted population, open) | this paper | EMD-9312 |
| Coordinates BMC-T four positions (TDTSTSTS) | this paper | PDB ID 6N0F |
| Map BMC-T four positions (TDTSTSTS) | this paper | EMD-9314 |
| Coordinates BMC-T four positions (TDTDTDTD) | this paper | PDB ID 6N09 |
| Map BMC-T four positions (TDTDTDTD) | this paper | EMD-9313 |
| Coordinates BMC-T four positions (TSTDTDTD) | this paper | PDB ID 6N0G |
| Map BMC-T four positions (TSTDTDTD) | this paper | EMD-9315 |
| Map HO shell C1 refinement | this paper | EMD-9296 |
| Coordinates HO shell X-ray structure | (Sutter et al., 2017) | PDB ID 5V74 |
| Map HO shell low-resolution cryo-EM (initial reference) | (Sutter et al., 2017) | EMD-8747 |
| Coordinates of ligand-bound CcmP, fully open | (Larsson et al., 2017) | PDB ID 5LT5 |
| Coordinates of BMC-TS from Citrobacter freundii | (Pang et al., 2011) | PDB ID 3PAC |
| Coordinates of BMC-TS from Haliangium ochraceum | (Aussignargues et al., 2016) | PDB ID 5DIH |
| Software and Algorithms | ||
| MOTIONCOR2 | (Zheng et al., 2017) | N/A |
| CTFFIND 4 | (Rohou and Grigorieff, 2015) | N/A |
| LEGINON | (Suloway et al., 2005) | N/A |
| RELION 1.4 | (Scheres, 2012) | N/A |
| RELION 2.0 | (Kimanius et al., 2016) | N/A |
| FREALIGN 9 | (Grigorieff, 2007; Grigorieff, 2016) | N/A |
| PHENIX 1.14 | (Adams et al., 2010) | N/A |
| COOT 0.8.1 | (Emsley et al., 2010) | N/A |
| HMMSCAN | (Eddy, 2008) | N/A |
| CLUSTAL X | (Larkin et al., 2007) | N/A |
| HOMOLMAPPER | (Rockwell and Lagarias, 2007) | N/A |
| UCSF CHIMERA 1.11 | (Pettersen et al., 2004) | N/A |
| PYMOL 1.7 | Schrödinger, LLC | N/A |
| Other | ||
| C-flat 1.2/1.3 holey carbon films on Cu 400 mesh grid | Protochips, Morrisville, USA | Cat# CF-1.2/1.3–4C |