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Lane 1: molecular weight marker (size indicated in KDa), Lane 2: un-induced sample, Lane 3: sample induced with 0.5 mM IPTG, Lane 4: protein lysate, Lanes 5 and 6: flow-through, Lane 7: wash step, Lane 8: purified EstATII and Lane 9: western blot analysis of the purified EstATII.
