Table 3. Effect of Temperature, pH, Substrate Chain Length and Certain Additives on the EstATII Activity.
| Variable | Relative Activity |
|---|---|
| Temperature (°C) | |
| 30 | 56.3 ± 0.8 |
| 35 | 59.9 ± 1 |
| 40 | 65.3 ± 1.8 |
| 45 | 86.9 ± 0.9 |
| 50 | 90.9 ± 2 |
| 55 | 93.9 ± 1 |
| 60 | 95.9 ± 0.3 |
| 65a | 100 ± 0.2 |
| 70 | 91.6 ± 0.15 |
| 75 | 76.7 ± 0.3 |
| 80 | 46.7 ± 1.3 |
| pH | |
| 3 | 0 |
| 4 | 0 |
| 5 | 0 |
| 5.5 | 13.1 ± 0.2 |
| 6 | 38.5 ± 1.2 |
| 6.5 | 43.2 ± 2 |
| 7 | 81.5 ± 1 |
| 7.5 (Na Phosphate) | 88 ± 1 |
| 7.5 (Tris.HCl) | 66.1 ± 0.2 |
| 8 | 84.7 ± 1.1 |
| 8.5a | 100 ± 3 |
| 9 | 60 ± 2 |
| Substrate Specificity | |
| pNP-acetate (C2) | 100 ± 3 |
| pNP-butyrate (C4) | 61.9 ± 0.8 |
| pNP-valerate (C5) | 40.5 ± 1.2 |
| pNP-decanoate (C10) | 0 |
| pNP-dodecanoate (C12) | 0 |
| pNP-myristate (C14) | 0 |
| pNP-palmitate (C16) | 0 |
| Sodium Chlorideb | |
| 0.5 M | 102.2 ± 2 |
| 1 M | 99.9 ± 0.5 |
| 1.5 M | 120 ± 1 |
| 2 M | 122.9 ± 1 |
| 2.5 M | 102.4 ± 1.8 |
| 3 M | 73.8 ± 3.8 |
| 3.5 M | 65.3 ± 4.6 |
| 4 M | 53.8 ± 1 |
| 4.5 M | 39.7 ± 1.7 |
| Detergents (0.1%) | |
| SDS | 6.1 ± 0.4 |
| Triton X-100 | 42.2 ± 1.1 |
| Tween 20 | 87.5 ± 2.3 |
| Tween 80 | 100.7 ± 2.3 |
| Detergents (1%) | |
| SDS | 0 |
| Triton X-100 | 4.3 ± 1 |
| Tween 20 | 40.1 ± 0.9 |
| Tween 80 | 54.5 ± 0.7 |
| Reducing and Modifying Agents (1 mM) | |
| β-mercaptoethanol | 117.9 ± 0.3 |
| DTT | 78.9 ± 4.2 |
| DEPC | 48 |
| CTAB | 13.1 ± 1.9 |
aOptimum condition (defined as 100%).
bActivity in the absence of NaCl (defined as 100%).