Table 1. Mutation effect of GO on PKCζ gene that assessed by the clone experiment.
| Group | Experiment No. | Clone number | Total gene length (bp) | Mutation number (bp) | Mut. Ratea (‰) | Mean mut. rate (‰) |
|---|---|---|---|---|---|---|
| Control | 1 | 32 | 19200 | 7 | 0.36 | 0.38 |
| 2 | 32 | 19200 | 7 | 0.36 | ||
| 3 | 32 | 19200 | 8 | 0.42 | ||
| GO | 1 | 32 | 19200 | 10 | 0.52 | 0.52b |
| 2 | 32 | 19200 | 10 | 0.52 | ||
| 3 | 33 | 19800 | 10 | 0.51 |
aMut. rate (mutation rate) was calculated by dividing the mutation number observed by the total gene length.
bCompared to the control, GO-treatment group at 10 ng/mL showed statistically significant difference in mutation rate (p < 0.05).