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Dose response curves for regorafenib, erlotinib and 5-fluorouracil in 2D and 3D cultures. Metabolic activity dose-response curves for HCT116, SW620 and DLD1 2D and 3D cultures after 72 hours treatment with regorafenib, erlotinib or 5-fluorouracil. Error bars represent the standard deviation for n = 3–15. Significances of *p < 0.05 and **p < 0.01 represent the difference for each drug dose between 2D and 3D cultures, determined with the unpaired multiple T-test.
