Figure 7.

Detection of extracellular matrix components in the 3D and 3D co-cultures. (A,C,E) Extracellular matrix components laminin and fibronectin, as well as beta (β)-actin, were detected in control and treated 3D and 3D co-cultures of HCT116 (green), SW620 (red) DLD1 (blue) cells as indicated. (B,D,F) quantification of laminin protein expression of 3D and 3D-CC spheroids with and without 2.5% Matrigel^TM(BM). Results are presented as mean values of N = 2–3 independent experiments with the error bars representing the standard deviation. Significances of **p < 0.01, ***p < 0.001 were determined with a two-way ANOVA test with post-hoc Tukey’s multiple comparison. (G) Representative western blots quantified in (A–F). All gels for western blot analysis were run under the same experimental conditions. 50% indicates 3D co-cultures of CRC cells with fibroblasts in ratio 1:1 and 5% endothelial cells. Unprocessed full blots can be found in Supplementary Fig. S10. The blots images were prepared in compliance with the digital image and integrity policies of the journal. Images were obtained with the Licor Odyssey CLx scanner at one default exposure setting.