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. 2019 May 8;10(5):369. doi: 10.1038/s41419-019-1603-4

Fig. 1. Immunohistochemistry (IHC), western blotting (WB), and quantitative real-time PCR (qPCR) characteristics of NCKAP1 in hepatocellular carcinoma (HCC) specimens. NCKAP1 was primarily expressed in the tumor cell cytoplasm.

Fig. 1

a Representative images of negative NCKAP1 expression in the tumor cytoplasm (×200). The lower panel shows an enlargement of the indicated area (×400). b Representative staining of negative NCKAP1 expression in the tumor cell cytoplasm (×200). The lower panel shows an enlargement of the indicated area (×400). c WB results show that NCKAP1 exhibited higher expression in tumor tissues compared to adjacent normal tissue in patient samples. d qPCR confirms the higher expression of NCKAP1 in tumor tissues