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. 2019 May 8;10(5):369. doi: 10.1038/s41419-019-1603-4

Fig. 3. NCKAP1 expression in a normal liver cell line and hepatocellular carcinoma (HCC) cell lines.

Fig. 3

a Western blotting results show that L02, SMMC-7721, and SK-Hep-1 cells exhibited low expression compared to that of Hep-3B and Huh-7 cells. GAPDH was used as a control. b Quantitative real-time PCR (qPCR) results confirmed the high expression of NCKAP1 in Hep-3B and Huh-7 cells. c Overexpression of NCKAP1 (OE) in a transfected SMMC-7721 cell line verified by western blotting and qPCR compared to that of cells transfected with the control vector (Vec). GAPDH was used as a control. d Overexpression of NCKAP1 in a transfected SK-Hep-1 cell line verified by western blotting and qPCR. GAPDH was used as a control