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. 2019 May 8;9:7090. doi: 10.1038/s41598-019-43564-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunolocalization of vimentin (a–d) and PCNA (e–h) in the renal cortex of control (a,e), control + calcitriol (b,f), glycerol (c,g) and glycerol + calcitriol (d,h) animals. The bar indicates 50 μm. Percentage of tubulointerstitial area in marked with vimentin (i) and number of PCNA⁺ cells (k) in the renal cortex. Western blot analysis of vimentin (j) and PCNA (l) in the renal cortex of control (lane 1), control + calcitriol (lane 2), glycerol (lane 3), and glycerol + calcitriol (lane 4) animals. The densitometric ratio between vimentin or PCNA and GAPDH was calculated, and the data are expressed compared with those of the control group, with the mean (±SEM) control value designated 100% and expressed as the means ± SEM. Blots are representative imagens from independent experiments. *Denotes a statistical significance of P < 0.05 between the groups. (n = 6–7 for each group). Magnification, ×400.