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. 2019 May 8;9:7090. doi: 10.1038/s41598-019-43564-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunolocalization of ED1⁺ cells (macrophages) in the renal cortex of control (a), control + calcitriol (b), glycerol (c) and glycerol + calcitriol (d) animals and number of ED1+ cells in the tubulointerstitial compartment (e). The bar indicates 50 μm. Levels of urinary MCP-1 (f) evaluated by ELISA of animals from different groups. Western blot analysis of caspase 3 (g) in the renal cortex of control (lane 1), control + calcitriol (lane 2), glycerol (lane 3) and glycerol + calcitriol (lane 4) animals. The densitometric ratio between caspase 3 and GAPDH was calculated, and the data are expressed compared with that of the control group, with the mean (±SEM) control value designated 100%. Blots are representative images from independent experiments. The data are expressed as the means ± SEM. *Denotes a statistical significance of P < 0.05 between the groups. (n = 7–12 per group). Magnification, ×400.