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. 2019 May 8;9:7090. doi: 10.1038/s41598-019-43564-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunolocalization of NF-κB (a–d) and p-JNK (e–h) in the renal cortex of control (a,e), control + calcitriol (b,f), glycerol (c,g) and glycerol + calcitriol (d,h) animals. The bar indicates 50 μm. Inset: slices without counterstaining. Percentage of the tubulointerstitial area of the renal cortex marked with NF-κB (i) and p-JNK (j) in the animals from different groups. Levels of IL-1β in the renal cortex (k) evaluated by ELISA of animals from different groups. The data are expressed as the means ± SEM. *Denotes a statistical significance of P < 0.05 between the groups. (n = 7–12 per group). Magnification, ×400; inset magnification, ×840.