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Advantages and disadvantages of three common EVs separation methods
| Method | Advantages | Disadvantages |
|---|---|---|
| Ultracentrifugation | Low cost;simple operation | Time-consuming; easy to be polluted; low yield |
| Immunoisolation | More specific in capturing a small amount of plasma EVs | Quite expensive;not suitable for large-scale; |
| Ultrafiltration | A small amount of sample can also get enough quantity | Cause some vesicles to deform or rupture |
