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. 2019 May 8;9:7089. doi: 10.1038/s41598-019-43607-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gating of microvesicles. The respective antibody was added to CSF, incubated and measured directly and undiluted with flow cytometry. Microvesicles were defined by a gate that excluded background and particles with a higher forward scatter than 1 µm beads. The expression of CD3, CD4, CD45, CD64, Aβ, APP, BACE1, and tau was analyzed within the microvesicle gate (MV < 1 µm). Gates defining the respective antigen positive fraction were drawn in respect to an obviously antigen negative fraction within the same measurement.