Table 2.
Intraassay CV | Interassay CV | Interassay CV | Inter-Alliquot CV | |
---|---|---|---|---|
without background normalization | with background normalization |
|||
replications | 5 | 5 | 5 | 5 |
number MV | 2.1% | 42.3% | n.d. | 24.5% |
Tau (MFI) | 15.4% | 7.3% | 28.03% | 12.0% |
APP (MFI) | 3.3% | 26.0% | 33.09% | 22.6% |
Aβ (MFI) | 9.4% | 125.2% | 128.89% | 41.3% |
CD3 (MFI) | 4.5% | 18.6% | 30.8% | 25.8% |
CD4 (MFI) | 4.2% | 28.5% | 39.82% | 6.7% |
CV: coefficient of variation, MV: microvesicles; MFI: mean fluorescent intensity.