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. 2019 May 8;9:7084. doi: 10.1038/s41598-019-43572-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dynamic monitoring of CTC count and their prognostic value for recurrence. (A) Patient A with increased CTC number, biophenotypic and mesenchymal CTC proportion showed HCC recurrence. (B) Patient B was diagnosed with HCC recurrence 4 months after the last CTC test. (C) Patient C was diagnosed with HCC recurrence with continuous decreases of CTC number. (D) CTC count increased continuously and biophenotypic and mesenchymal CTC accounted for most of the CTC in patient D who without recurrence. (E,F) In patient E and patient F, CTC count and proportion of biophenotypic and mesenchymal CTC increased and declined irregularly and no HCC recurrence observed.