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. 2019 May 8;10:2105. doi: 10.1038/s41467-019-09807-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — RSV F trimers open and dissociate at the cell surface. a Full-length RSV F proteins were expressed on the surface of FreeStyle 293-F cells and incubated with either CR9501 Fab or motavizumab Fab. Cells were then stained with CF-647-labeled CR9501 IgG or CF-647-labeled motavizumab IgG and binding was analyzed by flow cytometry. b A cartoon depicts prefusion F protomers in blue, light gray, and gray and shows four possible states of the prefusion F trimer, two of which could allow simultaneous binding of motavizumab and CR9501. c Full-length RSV F or a variant in which the cytoplasmic tail was replaced with a Foldon trimerization motif was expressed on the surface of FreeStyle 293-F cells and stained as in (a). d Same as (c), but with RSV F and the Foldon variant expressed on the surface of HeLa cells. Bar graphs show the mean and error bars indicate the standard deviation (n = 3 biologically independent experiments with two technical replicates)