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. 2019 May 8;9:7096. doi: 10.1038/s41598-019-43275-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Thrombocytopenia and platelet apoptosis in patients with dengue infection or HIV plus dengue coinfection: (A) Prospective analysis of platelet counts at the febrile, defervescence and recovery phase of dengue illness in patients infected with dengue only or coinfected with HIV plus dengue (HIV + dengue); (B) The percentage of phosphatidylserine exposure measured by the binding of Annexin V on platelets from patients with dengue or HIV + dengue; (C) The fluorescence of the probe TMRE indicating the mitochondrial membrane potential (Δψ_m) in platelets from patients with dengue or HIV + dengue; and (D) The fluorescence of the probe FLICA indicating active caspase-9 in platelets obtained at acute and recovery phase from patients with dengue or HIV + dengue. The horizontal lines in the box plots represent the median, the box edges represent the interquartile ranges and the whiskers indicate 5–95 percentile. *Signifies p < 0.05 compared to patients with the same profile of infection (dengue or HIV + dengue) at the recovery.