Figure 5.

SBF2-AS1-miR-338-3p/362-3p-E2F1 Axis Promotes LUAD Tumorigenesis

E2F1 protein level decreased as SBF2-AS1 was knocked down and increased as SBF2-AS1 was overexpressed (A). Expression of SBF2-AS1 and expression of E2F1 were positively correlated in LUAD tissues (p = 0.0001, R² = 0.772) (B). E2F1 protein level was decreased by miR-338-3p and miR-362-3p and could be restored by SBF2-AS2, but after mutation of miR-338-3p (C) or miR-362-3p (D) binding sites, the SBF2-AS1-MUT could not restore E2F1 expression (C and D). Overexpression of SBF2-AS1 increased A549 cell proliferation activity, while the increase could be partially reversed by both miR-338-3p and miR-362-3p, and co-administration of miR-338-3p and miR-362-3p completely abolished the promoting effect driven by SBF2-AS1; colony formation assay was used in (E), and EdU assay was used in (F). *p < 0.05, **p < 0.01; N.S, no statistical significance. Error bars stand for mean and SE.