Figure 3.

AIP Regulates GC Organization and AKT Signaling in GC B Cells

(A) Expression of dark zone (DZ) (CXCR4⁺) and light zone (LZ) (CD86⁺) GC B cells from immunized Aip^fl/fl Cre⁺ and littermate controls.

(B) Ratio of DZ and LZ GC B cells f.

(C) Spleen sections from Aip^fl/fl Cre⁺ and Cre⁻ mice were analyzed by immunofluorescence for LZ CD19⁺ BCL6⁺ and DZ AID⁺ BCL6⁺ areas of GCs.

(D–F) The phosphorylation of AKT (serine 473) was determined in LZ GC B cells from Aip^fl/fl Cre⁺ and littermate controls (D) and the percentage of pAKT (E) and MFI (F) determined. Grey histograms are gating on IgD⁺ naive B cells used as a biological control.

(G and H) IgD⁻ B cells from Cre⁺ and Cre⁻ mice were stimulated with anti-IgM (10 μg/mL) and examined for the expression of phosphorylated AKT from 5 to 60 min post-stimulation (G) and the percentage increase from time zero determined (H). See also Figure S6. Grey histograms show expression at time zero (T0).

Scale bars, 50 μm. Results are from two or three independent experiments with one or two mice per experimental group. ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001.