Figure 4.

AIP Regulates GC B Cells Independently of T Cells

(A and B) GC B cells (GL7⁺ CD95⁺) from Aip^fl/fl mice crossed with Cγ1^Cre/+ mice and examined at baseline (open circles) or following immunization with 2 × 10⁹ SRBCs (filled circles) 10 days later (A) and the expression of BCL6 was assessed by flow cytometry (B).

(C and D) The percentage of GC B cells (C) and MFI from baseline (unimmunized; D) and immunized Aip^fl/fl; Cγ1^Cre/+ mice was determined.

(E) Expression and ratio of DZ (CXCR4⁺) and LZ (CD86⁺) GC B cells from immunized mice.

(F) Serum anti-SRBC IgG determined by incubating serially diluting serum from Cre⁻ and Cre⁺ mice.

Results are from two independent experiments with three mice per group. ^∗p < 0.05; ^∗∗p < 0.01.