REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
CD16/32 Supernatant harvested from hybridoma | Raulet Lab, UC, Berkeley | N/A |
B220 FITC clone RA3-6B2 | Biolegend | AB_312990 |
CD4 APC clone GK1.5 | Biolegend | AB_312696 |
CD3e AF700 clone145-2C11 | Thermo Fisher Scientific (eBioscience) | AB_837094 |
CD21/35 clone eBio8D9 | Thermo Fisher Scientific (eBioscience) | AB_10855041 |
CD23 PE-Cy7 clone B3B4 | Biolegend | AB_2103037 |
CD38 BV421 clone 90 | Thermo Fisher Scientific (eBioscience) | AB_11218302 |
CD95 PE clone 15A7 | Thermo Fisher Scientific (eBioscience) | AB_465788 |
CD83 eFluor450 clone Michel-19 | Biolegend | AB_2566123 |
CD138 PE clone 281-2 | Biolegend | AB_10915989 |
IgD APC clone11-26c | Thermo Fisher Scientific (eBioscience) | AB_10598660 |
IgM eFluor 450 clone eB121-15F9 | Thermo Fisher Scientific (eBioscience) | AB_10671539 |
CD24 APC clone M1/69 | Thermo Fisher Scientific (eBioscience) | AB_2534261 |
CD43 PE clone eBioR2/60 | Thermo Fisher Scientific (eBioscience) | AB_465659 |
CXCR4 PerCP clone L276F12 | Biolegend | AB_2562786 |
Rat anti CD19 eFlour660 clone eBio1D3 | Thermo Fisher Scientific (eBioscience) | AB_657650 |
CD86 PE-Cy7 clone GL1 | Thermo Fisher Scientific (eBioscience) | AB_2573372 |
GL-7 Pacific Blue clone GL-7 | Biolegend | AB_2563291 |
AIP | Novus Biologicals | AB_10002466 |
BCL6 PerCP clone BCL-DWN | Thermo Fisher Scientific (eBioscience) | AB_2573767 |
BCL6 | CST | AB_10949970 |
FBXO11 | Bethyl Laboratories | AB_890603 |
UCHL1 | Proteintech | AB_2210497 |
pAKT eFluor 450 clone SDRNR | Thermo (eBioscience) | AB_2574125 |
p-ERK PE | Thermo Fisher Scientific (eBioscience) | AB_2572695 |
p-SYK PE | Thermo Fisher Scientific (eBioscience) | AB_2572675 |
Anti-mouse IgG biotin | Thermo Fisher Scientific (eBioscience) | AB_466650 |
Anti-mouse IgM | Jackson Laboratory | AB_2340761 |
Anti-mouse CD40 clone 1C10 | Biolegend | AB_312942 |
Rabbit anti BCL6 clone N-3 | Santa Cruz | AB_2063450 |
Rat anti-mouse IgD APC clone 11-26c.2a | BD Biosciences | AB_10612002 |
Anti-mouse IgM FITC | Southern Biotech | AB_2687524 |
Goat goat anti-mouse IgG1 Alexa Flour 647 | Life Technologies | AB_141658 |
Goat anti-mouse IgG2a Alexa Flour 647 | Life Technologies | AB_141698 |
Bacterial and Virus Strains | ||
pHIV1 lentivirus plasmid | Barts Cancer Institute | N/A |
pVSV-G lentivirus plasmid | Barts Cancer Institute | N/A |
Biological Samples | ||
Human DLBCL tissue microarray for 33 patients | Barts Cancer Institute Tissue Bank | N/A |
Chemicals, Peptides, and Recombinant Proteins | ||
Sheep Red Blood Cells | TSC Biosciences | Cat # SB069 |
NP-PE | Biosearch Technologies | Cat # N-5070-1 |
NP-BSA | Biosearch Technologies | Cat # N-5050XL-10 |
NP-Keyhole Limpet Hemocyanin | Biosearch Technologies | Cat # N-5060-5 |
Recombinant mouse IL-4 | Peprotech | Cat # 214-14 |
Experimental Models: Cell Lines | ||
HEK293T | ATCC | CRL-3216 |
OC1-LY7 | ATCC | ACC688 |
SUDHL6 | Barts Cancer Institute | N/A |
OC1-LY1 | Barts Cancer Institute | N/A |
DoHH2 | Barts Cancer Institute | N/A |
Karpas422 | Barts Cancer Institute | N/A |
A20 | Barts Cancer Institute | N/A |
EBV of healthy control | Korbonits Lab (Hernández-Ramírez et al., 2016) | N/A |
Experimental Models: Organisms/Strains | ||
Aip flox/flox mice | Jackson Laboratories | 013195 |
Ahr flox/flox mice | Stockinger Lab, Crick Institute, London (Li et al., 2011) | N/A |
Rag1 Cre mice | Stockinger Lab, Crick Institute, London (Li et al., 2011) | N/A |
Cγ1 Cre mice | Calado Lab, Crick Institute, London (Calado et al., 2012) | N/A |
Oligonucleotides | ||
Rag1 KI CRE F: 5′-TTTGTTTTTGTTTGCTTGTTTGA | Sigma | N/A |
Rag1 WT R: 5′-ATCCTTCTCCTTCTGTGCTTCTT | Sigma | N/A |
Rag1 KI CRE (V2): 5′-AATGTTGCTGGATAGTTT TTACTGC |
Sigma | N/A |
Cγ1 IgG1 Kpn1 (WT): 5′ -TGTTGGGACAAACG AGCAATC |
Sigma | N/A |
Cγ1 Cre Cre13 (CRE) 5′-GGTGGCTGGACCAA TGTAAATA |
Sigma | N/A |
Cγ1 Cre IgG1Rev (Common) 5′-GTCATGGCAAT GCCAAGGTCGCTAG |
Sigma | N/A |
Aip: F 5′-CAATCCCCCACTGTCACTT | Sigma | N/A |
Aip: R- 5′-TCACCCCTCCCACTGACTAC | Sigma | N/A |
Aip Smart vector lentiviral shRNA | Dharmacon | N/A |
Recombinant DNA | ||
AIP-MYC pcDNA3.1 | Korbonits Lab (Leontiou et al., 2008) | N/A |
BCL6-FLAG pcDNA3.1 | SinoBiological | Cat # NM_138931 |
FBXO11-FLAG pCMV3 | SinoBiological | Cat # BC130445 |
Ub-HA pRK5 | Nightingale Lab (QMUL, London) | N/A |
UCHL1-FLAG | SinoBiological | Cat # NM_004181.4 |
Software and Algorithms | ||
PRISM version 6 | Graphpad.com | N/A |
FlowJo Version 9.3.1 | Tree Star | N/A |
Ariol Software | Leica Biosystems | N/A |