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. 2019 Apr 30;27(5):1461–1471.e4. doi: 10.1016/j.celrep.2019.04.014
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

CD16/32 Supernatant harvested from hybridoma Raulet Lab, UC, Berkeley N/A
B220 FITC clone RA3-6B2 Biolegend AB_312990
CD4 APC clone GK1.5 Biolegend AB_312696
CD3e AF700 clone145-2C11 Thermo Fisher Scientific (eBioscience) AB_837094
CD21/35 clone eBio8D9 Thermo Fisher Scientific (eBioscience) AB_10855041
CD23 PE-Cy7 clone B3B4 Biolegend AB_2103037
CD38 BV421 clone 90 Thermo Fisher Scientific (eBioscience) AB_11218302
CD95 PE clone 15A7 Thermo Fisher Scientific (eBioscience) AB_465788
CD83 eFluor450 clone Michel-19 Biolegend AB_2566123
CD138 PE clone 281-2 Biolegend AB_10915989
IgD APC clone11-26c Thermo Fisher Scientific (eBioscience) AB_10598660
IgM eFluor 450 clone eB121-15F9 Thermo Fisher Scientific (eBioscience) AB_10671539
CD24 APC clone M1/69 Thermo Fisher Scientific (eBioscience) AB_2534261
CD43 PE clone eBioR2/60 Thermo Fisher Scientific (eBioscience) AB_465659
CXCR4 PerCP clone L276F12 Biolegend AB_2562786
Rat anti CD19 eFlour660 clone eBio1D3 Thermo Fisher Scientific (eBioscience) AB_657650
CD86 PE-Cy7 clone GL1 Thermo Fisher Scientific (eBioscience) AB_2573372
GL-7 Pacific Blue clone GL-7 Biolegend AB_2563291
AIP Novus Biologicals AB_10002466
BCL6 PerCP clone BCL-DWN Thermo Fisher Scientific (eBioscience) AB_2573767
BCL6 CST AB_10949970
FBXO11 Bethyl Laboratories AB_890603
UCHL1 Proteintech AB_2210497
pAKT eFluor 450 clone SDRNR Thermo (eBioscience) AB_2574125
p-ERK PE Thermo Fisher Scientific (eBioscience) AB_2572695
p-SYK PE Thermo Fisher Scientific (eBioscience) AB_2572675
Anti-mouse IgG biotin Thermo Fisher Scientific (eBioscience) AB_466650
Anti-mouse IgM Jackson Laboratory AB_2340761
Anti-mouse CD40 clone 1C10 Biolegend AB_312942
Rabbit anti BCL6 clone N-3 Santa Cruz AB_2063450
Rat anti-mouse IgD APC clone 11-26c.2a BD Biosciences AB_10612002
Anti-mouse IgM FITC Southern Biotech AB_2687524
Goat goat anti-mouse IgG1 Alexa Flour 647 Life Technologies AB_141658
Goat anti-mouse IgG2a Alexa Flour 647 Life Technologies AB_141698

Bacterial and Virus Strains

pHIV1 lentivirus plasmid Barts Cancer Institute N/A
pVSV-G lentivirus plasmid Barts Cancer Institute N/A
Biological Samples
Human DLBCL tissue microarray for 33 patients Barts Cancer Institute Tissue Bank N/A

Chemicals, Peptides, and Recombinant Proteins

Sheep Red Blood Cells TSC Biosciences Cat # SB069
NP-PE Biosearch Technologies Cat # N-5070-1
NP-BSA Biosearch Technologies Cat # N-5050XL-10
NP-Keyhole Limpet Hemocyanin Biosearch Technologies Cat # N-5060-5
Recombinant mouse IL-4 Peprotech Cat # 214-14

Experimental Models: Cell Lines

HEK293T ATCC CRL-3216
OC1-LY7 ATCC ACC688
SUDHL6 Barts Cancer Institute N/A
OC1-LY1 Barts Cancer Institute N/A
DoHH2 Barts Cancer Institute N/A
Karpas422 Barts Cancer Institute N/A
A20 Barts Cancer Institute N/A
EBV of healthy control Korbonits Lab (Hernández-Ramírez et al., 2016) N/A

Experimental Models: Organisms/Strains

Aip flox/flox mice Jackson Laboratories 013195
Ahr flox/flox mice Stockinger Lab, Crick Institute, London (Li et al., 2011) N/A
Rag1 Cre mice Stockinger Lab, Crick Institute, London (Li et al., 2011) N/A
Cγ1 Cre mice Calado Lab, Crick Institute, London (Calado et al., 2012) N/A

Oligonucleotides

Rag1 KI CRE F: 5′-TTTGTTTTTGTTTGCTTGTTTGA Sigma N/A
Rag1 WT R: 5′-ATCCTTCTCCTTCTGTGCTTCTT Sigma N/A
Rag1 KI CRE (V2): 5′-AATGTTGCTGGATAGTTT
TTACTGC
Sigma N/A
Cγ1 IgG1 Kpn1 (WT): 5′ -TGTTGGGACAAACG
AGCAATC
Sigma N/A
1 Cre Cre13 (CRE) 5′-GGTGGCTGGACCAA
TGTAAATA
Sigma N/A
Cγ1 Cre IgG1Rev (Common) 5′-GTCATGGCAAT
GCCAAGGTCGCTAG
Sigma N/A
Aip: F 5′-CAATCCCCCACTGTCACTT Sigma N/A
Aip: R- 5′-TCACCCCTCCCACTGACTAC Sigma N/A
Aip Smart vector lentiviral shRNA Dharmacon N/A

Recombinant DNA

AIP-MYC pcDNA3.1 Korbonits Lab (Leontiou et al., 2008) N/A
BCL6-FLAG pcDNA3.1 SinoBiological Cat # NM_138931
FBXO11-FLAG pCMV3 SinoBiological Cat # BC130445
Ub-HA pRK5 Nightingale Lab (QMUL, London) N/A
UCHL1-FLAG SinoBiological Cat # NM_004181.4

Software and Algorithms

PRISM version 6 Graphpad.com N/A
FlowJo Version 9.3.1 Tree Star N/A
Ariol Software Leica Biosystems N/A