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. 2019 May 6;21(6):602–614. doi: 10.1016/j.neo.2019.03.008

Figure 3.

Figure 3

ALDH2 expression inhibits the stem-like features of lung adenocarcinoma cells.

A. Identification of side population and main population in A549-GFP, A549-ALDH2 cells by FACS using Ko143 (5 μM) as an inhibitor of ABCG2 binding cassette. B. FACS analysis of the CD44+/C24- population of A549-GFP and A549-ALDH2 cells. C. FACS analysis of the CD44+/C24- population of H1299-GFP and H1299-ALDH2 cells. D. ROS analysis of A549, A549-ALDH2 cells by FACS. E. ROS analysis of H1299, H1299-ALDH2 cells by FACS. F. ROS analysis of H1299, H1299-ALDH2 cells treated with ACE for 2 days by FACS. G. Western blot analysis of CD44 in H1299-GFP cells, H1299-ALDH2 cells, H1792-shNS cells and H1792-shALDH2 cells.