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. 2019 May 6;21(6):602–614. doi: 10.1016/j.neo.2019.03.008

Figure 4.

Figure 4

ALDH2-deficiency leads to accumulated ACE and increased DNA damage.

A. ACE quantification of A549-shNS and A549-shALDH2 cells by Mass spectrometry, *P < .05. B. Comet assay of A549-GFP and A549-ALDH2 cells that were treated with or without 4 mM ACE for 2 days. C. Western blot assay of γH2AX and ALDH2 in A549-GFP and A549-ALDH2 cells that were treated with or without 4 mM ACE for 2 days. D. Western blot assay of γH2AX and ALDH2 in H1299-GFP and H1299-ALDH2 cells that were treated with or without 4 mM ACE for 2 days. E. Relative quantification of ACE by Mass spectrometry of WT and Aldh2-KO mice lung tissues that were intraperitoneally injected with or without Ethanol (28% v/v in saline). Results are the mean (S.E.M.) of triplicate samples. t Test P < .0001. F. Western blot assay of γH2AX and ALDH2 in lung tissues of WT and Aldh2-KO mice. G. IHC staining and the score count of γH2AX in lung tissues from WT and Aldh2-KO mice. H. HE staining of lung tissues from WT and Aldh2-KO mice. I. Correlation analysis of ALDH2 expression and mutation burden in lung adenocarcinoma tissues using TCGA data.