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. 2019 May 6;21(6):602–614. doi: 10.1016/j.neo.2019.03.008

Figure 6.

Figure 6

ALDH2 agonist Alda-1 alleviates malignant features of lung adenocarcinoma cells.

A. Identification of side population and main population in A549 cells that were treated with Alda-1for different concentrations (0 nM, 350 nM,5 μM) by FACS using Ko143 (5 μM) as an inhibitor of ABCG2 binding cassette. B. Identification of CD44+/C24- population in H1299 cells treated with Alda-1 for different concentrations (0 nM, 100 nM, 1 μM) by FACS analysis. C. Representative images and quantitative analysis of colonies formed from A549 cells that were treated with different concentrations of Alda-1 (0 nM, 100 nM, 1 μM) in soft agarose. Results are the mean (S.E.M.) of triplicate samples. D. Representative images and quantitative analysis of 3D-spheres formed from H1299-GFP and H1299-ALDH2 cells that were treated with or without 1 μM Alda-1. Results are the mean (S.E.M.) of triplicate samples. E. The migration behavior of A549-GFP and A549-ALDH2 cells that were treated with different concentrations of Alda-1 (0 nM, 350 nM, 1 μM) was analyzed by a wound healing assay.