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. Author manuscript; available in PMC: 2019 Nov 7.
Published in final edited form as: Pharm Res. 2018 Nov 7;36(1):3. doi: 10.1007/s11095-018-2541-z

Figure 2: Increased drug loading results in macrophages becoming concentrated within granulomas, and increased drug accumulation mainly within the granuloma.

Figure 2:

A-D) Untreated, 2, 4 and 8 week immunofluorescence images of liver cryosections stained for the macrophage marker F4/80 (green) and CLDI fluorescence (red). E) The total percentage of F4/80 or Cy5 fluorescence signal coming from the granuloma compared to the non-granulomatous tissue area was determined, revealing a significant increase from four to eight weeks of treatment. Yellow arrows point to granuloma macrophages loaded with CLDIs, while white arrows point to Kupffer cells with CLDIs. Scale bar is 100 µm. (n=3 animals per time point, 10 images per liver cryosections) (*=p<0.01, ANOVA, Tukey’s HSD, #=p<0.001, ANOVA, Tukey’s HSD).