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. Author manuscript; available in PMC: 2019 Nov 7.
Published in final edited form as: Pharm Res. 2018 Nov 7;36(1):3. doi: 10.1007/s11095-018-2541-z

Figure 3: Granuloma-associated macrophages are phenotypically different from both untreated and drug treated macrophages.

Figure 3:

A) Images showing untreated Kupffer cells, drug-treated macrophages and granuloma-associated macrophages isolated from 8 week CFZ-treated liver stained for TFEB, LAMP1 and LC3. B) Granuloma-associated macrophages show significantly elevated TFEB translocation to nucleus compared to untreated and drug-treated macrophages. C) Granuloma-associated macrophages are significantly larger than their untreated and drug-treated macrophage counterparts. D) Granuloma-associated macrophages show significantly elevated expression of LAMP1 within the cell, indicating increased lysosomal content within the cell. E) Granuloma-associated macrophages show increased punctate LC3 staining within the cytoplasm of the cell compared to untreated and drug-treated macrophages, indicating increased autophagic flux within the cell, which may be necessary to form new membranes to trap the CLDI. Scale bar is 15 um. (n=30-35 cells per group, *=p<0.05, ANOVA, Tukey’s HSD).