Fig. 2.

Co-segregation between 1,2RhaT and naringin accumulation in the progeny of ‘Red tangerine’ × ‘Trifoliata orange’ (Citrus reticulata × Poncirus trifoliata). (A) Naringin is the most abundant and representative neohesperidoside in citrus fruit and was detected in mature fruits of ‘Trifoliata orange’ but not in those of ‘Red tangerine’. (B) A DNA fragment of 1,2RhaT could be amplified from ‘Trifoliata orange’ (TO) but not from ‘Red tangerine’ (RT) using PCR primers 5´-GTGGATTATTGCTCAGCGA-3´ and 5´-ATTGGTACCCCGAAAACCAT-3´. (C) Strong co-segregation was detected between the presence of 1,2RhaT and naringin in F₁ progeny (individuals numbered 1–109). The DNA ladders are 100 bp.