Table 1.
Glucosyltransferase activity of 1,2RhaT and dGlcTs for different flavonoid substrates
| Subclass | Substrate applied | Structure | Substituent group | BY2-CitdGlcT-1 and BY2-CitdGlcT-2 | BY2-Cit1,2RhaT | |||
|---|---|---|---|---|---|---|---|---|
| Reaction product | Net amount (μg)1 | Reaction product | Net amount(μg)1 | |||||
| CitdGlcT-1 | CitdGlcT-2 | |||||||
| Flavanone |
Naringenin Hesperetin Nar-7-O-Glu |
|
R1=H, R2=H, R3=H, R4=H R1=H, R2=H, R3=OH, R4=CH3 R1=Glu, R2=H, R3=H, R4=H |
Nar-7-O-di-Glu (m/z 597.18) Hes-7-O-di-Glu (m/z 627.19) Nar-7-O-di-Glu (m/z 597.18) |
137.09±5.80a 112.84±0.55b 103.48±7.14c |
201.88±49.23a 135.54±5.20b 141.31±18.04b |
Naringin (m/z 581.19) Hesperedin (m/z 611.20) Naringin (m/z 581.19) |
406.54±1.41b 415.49±11.31a 371.61±18.67c |
| Flavone |
Apigenin Luteolin Diosmetin |
|
R1=H, R2=H, R3=H R1=OH, R2=H, R3=H R1=OH, R2=H, R3=CH3 |
Api-7-O-di-Glu (m/z 595.17) Lut-7-O-di-Glu (m/z 611.16) Dio-7-di-O-Glu (m/z 625.26) |
43.88±0.61d 30.99±0.64e 6.41±0.46gh |
51.59±3.58c 7.22±0.07f 21.69±0.92d |
Api-7-O-Neo (m/z 579.17) Lut-7-O-Neo (m/z 595.16) Dio-7-O-Neo (m/z 609.16) |
327.56±6.67d 229.41±6.88f 270.36±4.11e |
| Flavonol |
Kaempferol Quercetin Kael-3-O-Glu Kae-7-O-Glu Que-3-O-Glu Que-7-O-Glu |
|
R1=H, R2=H, R3=H, R4=H R1=H, R2=H, R3=OH, R4=H R1=H, R2=Glu, R3=H, R4=H R1=Glu, R2=H, R3=H, R4=H R1=H, R2=Glu, R3=OH, R4=H R1=Glu, R2=H, R3=OH, R4=H |
Kae-7-O-di-Glu (m/z 611.16) Que-7-O-di-Glu (m/z 627.16) Kae-3-O-di-Glu (m/z 611.16) Kae-7-O-di-Glu (m/z 611.16) Que-3-O-di-Glu (m/z 627.16) Que-7-O-di-Glu (m/z 627.16) |
2.41±0.13i 8.68±0.10fg 1.46±0.38i 1.67±0.04i 1.75±0.15i 9.23±0.76f |
2.42±0.98g 10.58±4.52ef 0.31±0.26g 0.58±0.24g 1.56±0.05g 10.95±0.44e |
Kae-7-O-Neo (None detected) Que-7-O-Neo (None detected) Kae-7-O-Neo (None detected) Kae-7-O-Neo (None detected) Que-7-O-Neo (None detected) Que-7-O-Neo (None detected) |
0 0 0 0 0 0 |
1Net amount of reaction products catalysed by 1,2RhaT or dGlcTs with 0.5 mg substrate. Data are means (±SD) of three biological replicates. Different letters indicate significant differences as determined using Duncan’s test (P<0.05). Net amount refers to the reaction product accumulation in the transgenic cell culture minus that in the wild-type cell culture.