Table 2.
Cell membrane injury in maize seedlings at low temperatures
| Electrolyte released (%) | |||||
|---|---|---|---|---|---|
| Inbred line | Temperature (°C) | 0 h | 24 h | 48 h | 72 h |
| B73 | −2 | 4.80±0.4 a |
17.88±1.27 de |
26.00±1.78 e |
49.25±2.44 e |
| +2 | 5.86±0.31 ab |
8.21±0.93 ab |
17.95±1.35 d |
27.13±1.72 c |
|
| +8 | 4.97±0.67 ab |
5.91±1.25 ab |
6.31±1.51 a |
5.04±1.34 a |
|
| S160 | −2 | 7.4±0.49 bcd |
29.18±1.81 f |
58.86±2.26 h |
61.35±2.49 f |
| +2 | 8.11±0.36 cd |
16.59±1.42 cde |
37.17±1.93 fg |
70.63±1.86 f |
|
| +8 | 8.31±0.85 bcd |
10.54±1.80 abcd |
11.96±2.32 abcd |
9.41±2.09 ab |
|
| S50676 | −2 | 5.98±0.44 ab |
11.38±1.06 bc |
28.64±19.42 ef |
43.09±2.42 de |
| +2 | 7.43±0.35 bcd |
7.97±0.9 ab |
9.30±1.00 ab |
13.09±1.21 b |
|
| +8 | 5.27±0.61 ab |
8.39±1.52 ab |
8.54±1.94 ab |
8.34±1.96 ab |
|
| S68911 | −2 | 5.79±0.41 ab |
21.00±1.48 e |
42.53±2.20 g |
48.99±2.44 e |
| +2 | 8.11±0.37 d |
8.22±0.85 ab |
16.67±1.34 cd |
28.41±1.58 c |
|
| +8 | 5.32±0.69 ab |
10.10±0.91 abcd |
8.41±1.93 ab |
7.33±1.82 ab |
|
| S84854 | −2 | 6.30±0.43 abcd |
11.37±1.1 bc |
32.25±1.82 ef |
33.59±2.21 cd |
| +2 | 6.46±0.32 abc |
5.83±0.75 a |
12.77±1.15 bcd |
13.64±12.23 b |
|
| +8 | 5.35±0.68 ab |
8.89±1.98 abc |
9.14±2.22 abc |
7.73±1.88 ab |
|
Mesocotyl fragments (2 cm long, three per sample) were cut from seedlings subjected to low temperatures for 1–3 d and electrolyte leakage following 150 min of shaking in water at room temperature was quantified. Data are normalized to amount of electrolyte released by boiling. Three independent experiments were conducted with up to 10 samples per experimental variant. Mean ±SE is shown. Within columns, different letters mark significant differences (P<0.05) estimated with the use of Beta Regression (betareg) in the R program.