Fig. 3.

Comparison of NMCP domain layout and the position of conserved motifs across the Spermatophyta. NMCPs present a tripartite domain layout, with a central CCD of highly conserved length (represented by bars) flanked by a short head domain and a longer tail domain. The tail domain is generally longer in NMCP1 than in NMCP2 proteins. Highly conserved motifs I–XI (MEME search) are located along the CCD and concentrated at the N-terminal end; motif I is characteristic for all NMCPs with the exception of a few algal sequences. The head and tail domains also contain highly conserved motifs specific to the NMCP family. Empty diamonds, GGLDEESLERKDRAALJAYI (motif 11 in Supplementary Fig. S2); filled diamonds, SWLRKCASKIF (motif 5 in Supplementary Fig. S2); thin upward arrows, phosphorylation sites; thick downward arrows, nuclear localization signal; thick upward arrows, QTPGEKRYNLRRSTIVNTVA (motif 14 in Fig. S2); four vertical bars, stretch of acidic amino acids; chevron arrows at C-terminus, EDEEPGEASIGKKLWNFLTT (motif 4 in Supplementary Fig. S2). Asterisks indicate conserved regions that were present in most but not all NMCPs analysed. Dicot NMCP1-type includes NMCP1 and NMCP3. (This figure is available in colour at JXB online.)