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. 2019 May 7;6(2):ENEURO.0434-18.2019. doi: 10.1523/ENEURO.0434-18.2019

Figure 2.

Figure 2.

Validation of Csmd2 antibodies. A, HEK293T cells were transfected with expression constructs for FLAG-tagged full-length (Csmd2 FL) or truncated (Csmd2 15x) Csmd2. The Novus α-Csmd2 antibody detected both full-length and truncated forms in Western blot analysis from whole cell lysates or after immunoprecipitation with α-FLAG antibody. SC-D18 and SC-G19 α-Csmd2 antibodies only detected the full-length construct. B, Fluorescence immunocytochemistry of HEK293T cells co-transfected with FLAG-Csmd2 full-length and myristoylated-tdTomato as a transfection marker. All three antibodies recognized exogenous Csmd2 in the transfected cells. C, As in B but stained with anti-FLAG antibody. Csmd2 (SC-G19) signal colocalized with FLAG signal at the plasma membrane. Scale bars, 10 µm.