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. 2019 May 7;6(2):ENEURO.0262-18.2019. doi: 10.1523/ENEURO.0262-18.2019

Figure 2.

Figure 2.

OTX2 in RMS and V-SVZ is non-cell-autonomous. A–C, Absence of Otx2 expression in V-SVZ (A, C) and RMS (B), as shown by GFP staining in coronal sections of Otx2+/GFP mice (A, B) and by in situ hybridization for Otx2 mRNA in coronal sections of wild-type mice (C). Note the expression of GFP in choroid plexus (A) and of Otx2 in septum (C). D, Representative sagittal section of RMS microdissection. E, Western blot (WB) analysis of OTX2 immunoprecipitation from lysates of various brain regions (ctrl, IgG control). Bands include OTX2 (<40 kDa), IgG (25 and 55 kDa), and unknown (∼45 kDa). SC, Superior colliculus; Str, striatum; M.W., molecular weight.