Fig. 6.

Subplastidial localization of carotenoid biosynthesis enzymes and biomarkers. (A) Abundance of carotenoid biosynthetic enzymes and biomarkers throughout the gradient. (B) PSY activity, (C) quantification of PSY protein abundance over two rounds of immunoblotting, and (D) PSY activity per amount of PSY-1 protein. Abundance was quantified using scanned images of the developed membranes in ImageJ. See Supplementary Table S6 for one-way ANOVA on PSY activity. Abbreviations: PSY, phytoene synthase; LCY, lycopene β-cyclase; β-CH, β-carotene hydroxylase; ZEP, zeaxanthin epoxidase; FIB, fibrillin; PGL, plastoglobulin; RbcL, Rubisco large subunit; Tic40, chloroplast inner envelope membrane translocon complex protein.