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. 2002 Jan;13(1):12–24. doi: 10.1091/mbc.01-04-0210

Figure 3.

Figure 3

Actin-binding site is of importance for neurite induction by full-length PKCε. (A) PKCε was modified by removing nucleotides encoding amino acids 223–228, i.e., the ABS, from wild-type PKCε cDNA (wt) and replacing it with an MluI recognition sequence, coding for amino acids T and R. This mutated PKCε (ΔABS) was also used to generate the new construct εRDΔABS. Neurite outgrowth was examined in SK-N-BE(2) (B) and SH-SY5Y cells (C) transfected with vectors encoding wild-type εFL and εRD (wt) and corresponding proteins with deleted actin-binding site (ΔABS), all fused to EGFP. The cells were fixed and mounted 16 h after transfection and transfected cells with long neurites were counted. Data (mean ± SEM, n = 3–6) are presented as percentage transfected cells with long neurites. ∗, statistically significant differences with analysis of variance followed by Duncan's multiple range test.