Figure 4.

GPC4 and GPC6 Are the Most Closely Related of All Glypican Proteins

(A) Phylogenetic analysis of vertebrate (2R) glypicans was inferred via the maximum-likelihood method. GPC1, GPC2, GPC4, and GPC6 share a common ancestor; GPC3 and GPC5 share a common ancestor. GPC4 and GPC6 have undergone considerably less diversification than have other glypican proteins. The tree is drawn to scale, and branch lengths are in the same units as those of the evolutionary distances used to infer the phylogenetic tree.

(B) The average amino acid identity and similarity (brackets) of vertebrate (2R) glypican proteins were determined by global pairwise alignment. Sequence accession numbers are indicated in Figure S1.