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. 2019 Apr 25;104(5):815–834. doi: 10.1016/j.ajhg.2019.03.022

Figure 2.

Figure 2

Generation of iPSC-Derived Neurons for BAF53 Studies

(A) Representative images of quality control staining done on iPSCs.

(B) Representative quality control staining on NPC cultures.

(C) Representative staining of control cells for TUJ1 and MAP2 at D15 of differentiation.

(D) Representative trace of miniature EPSCs from D25 neurons held at −40 mv.

(E) Representative recordings showing spontaneous activity of D25 neurons in current-clamp mode.

(F) Trace of a hyperpolarizing pulse showing a depolarizing sag followed by multiple rebound action potentials. The first action potential is shown at a higher temporal resolution.

All scale bars represent 40 μm.