Conductance trains from OVX+E PM GnRH neurons are most effective at initiating spikes in model neurons, but the feedback model also affects response. Individual values and mean ± SEM frequency during PSg trains prepared from OVX AM, OVX PM, OVX+E AM, and OVX+E PM mice in negative feedback (neg FB) and positive feedback (pos FB) model neurons. *p < 0.05, **p < 0.01, ***p < 0.0001: three-way repeated-measures ANOVA/Fisher's LSD test (PSg time-of-day: F(1,18) = 395, p < 0.001; PSg estradiol: F(1,18) = 2989, p < 0.001; PSg estradiol × time-of-day: F(1,18) = 910, p < 0.001; PSg time-of-day × feedback model: F(1,18) = 13.8, p = 0.002; PSg estradiol × feedback model: F(1,18) = 85.6, p < 0.001; PSg estradiol × time-of-day × feedback model: F(1,18) = 37.5, p < 0.001).