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. 2019 Mar 13;39(11):2080–2090. doi: 10.1523/JNEUROSCI.2701-18.2019

Figure 1.

Figure 1.

Chemogenetic approach. A, Schematic depicting dual viral system to activate (CRF-Cre + hSyn-DIO-hM3Dq) or inhibit (CRF-Cre + hSyn-DIO-hM4Di) CRF neurons in the PFC. B, Photomicrograph depicting mCherry expression in the caudal dmPFC from a CRF-hM3Dq-treated animal. Scale bar, 200 μm. cc, Corpus callosum. C, Top, Collapsed 30 μm z stack from inset in B, demonstrating mCherry colocalization with CRF-ir cells. Scale bar, 30 μm. Bottom, Inset from above, rotated at various angles. Scale bar, 10 μm. Di, CNO elicits an excitatory influence in PFC CRF neurons as measured by Fos-ir in mCherry neurons of CNO-treated CRF-hM3Dq animals. Dii, Absence of Fos-ir in the PFC of CNO-treated viral controls (mCherry + CNO; CRF-Cre hSyn-DIO-mCherry). Scale bar, 10 μm.