Fig. 4.

Transfection of lentivirus containing CCT6b gene to active fibroblasts significantly inhibited fibrous marker expressions. a Protein expressions of α-SMA and COL-1 in the IF-L group and IF group. b Relative mRNA expressions of α-SMA and COL-1 in the IF-L group compared to the IF group. The asterisk indicates p < 0.05 compared to the CF group. c Western blot results of CCT6b and CCT7 subunits in the IF and IF-L groups. d Relative quantitation of mRNA of CCT6b and CCT7 in the IF and IF-L groups was shown. IF fibroblasts derived from the immobilized knees, CF fibroblasts cells derived from the contralateral knees, IF-L fibroblasts derived from cells treated with overexpressed CCT6b lentivirus. GAPDH was used as the internal reference in the Western blot