Table 1.
Sequences mentioned in the article
| Name | Sequences | |
|---|---|---|
| Forward primer | Reverse primer | |
| CEACAM1 | 5′-GCTGGCATTGTGATTGGAGTA-3′ | 5′-TTAGGTGGGTCATTGGAGTG-3′ |
| CEACAM1-4S | 5′-AAGACGATCATAGTCACTGAGCT-3′ | 5′-ATTGGAGTGGTCCTGAGCTG-3′ |
| CEACAM1-4L | 5′-AAGACGATCATAGTCACTGAGCT-3′ | 5′-GGAGACTGAGGGTTTGTGCT-3′ |
| CEACAM1-3S | 5′-TCATAGTCACTGATAATGCTCTACC-3′ | 5′-ATTGGAGTGGTCCTGAGCTG-3′ |
| CEACAM1-3L | 5′-TCATAGTCACTGATAATGCTCTACC-3′ | 5′-GGAGACTGAGGGTTTGTGCT-3′ |
| CEACAM1-4C1 | 5′-AAGACGATCATAGTCACTGAGCT-3′ | 5′-TTGCACACCATTGACAGAGT-3′ |
| CEACAM1-3 | 5′-CAGTGACCCAGTCACCTTGA-3′ | 5′-TGGACTTGTTTGTGCCTGTTG-3′ |
| CEACAM1-3C2 | 5′-CAAGACGATCATAGTCACTGAGTC-3′ | 5′-AGAGGGACATATAGGAAGGGGT-3′ |
| PIK3CA | 5′-GGGATGATTTACGGCAAGATA-3′ | 5′-CCACACAGTCACCGATTGA-3′ |
| PIK3CB | 5′-TGCGACAGATGAGTGATGAA-3′ | 5′-TCCTCCGATTACCAAGTGCT-3′ |
| PIK3CD | 5′-GCCAACATCCAACTCAACAA-3′ | 5′-CCACACAATAGCCAGCACAG-3′ |
| PIK3CG | 5′-TAGACCACCGTTTCCTCCTG-3′ | 5′-CGTGAGTTTGTCAGCATTGA-3′ |
| PIK3C2A | 5′-GATTACCTGGGCCTTCCAC-3′ | 5′-AGTGGGCATTCTTGGATTGA-3′ |
| PIK3C2B | 5′-TCCACCTTGAACTACCTCGTC-3′ | 5′-AAGTCTCCATCAGCCAGCAG-3′ |
| PIK3C2G | 5′-CTCCTGGCATCAAGTTAGCA-3′ | 5′-TCTGGAATCATCAGCACCAT-3′ |
| PIK3C3 | 5′-ATCCCGTTGCCTTTAGAACC-3′ | 5′-TGCCTCCATCTTCCGTCTTA-3′ |
| PIK3R1 | 5′-GGACGGCGAAGTAAAGCAT-3′ | 5′-TGACATTGAGGGAGTCGTTG-3′ |
| PIK3R2 | 5′-GATGGGCACTATGGCTTCTC-3′ | 5′-TGCTGGTATTTGGACACAGG-3′ |
| PIK3R3 | 5′-AGCACAACGACTCCCTCAAC-3′ | 5′-AAATGCCAGAGAACCACCTC-3′ |
| PIK3R4 | 5′-TGGCATTTGTGTCCCTTTGT-3′ | 5′-TGCTGGATGAGTTGCTGAAG-3′ |
| PIK3R5 | 5′-TACACCACACTTCCCACCAG-3′ | 5′-CTCAGCCCTCACCAGTCTCT-3′ |
| PIK3R6 | 5′-TTCCCACTTCTCCCACTGTC-3′ | 5′-TGCTGTTCCTTGCTTCCAAT-3′ |
| IL-6 | 5′-GACAGCCACTCACCTCTTCAG-3′ | 5′-CATCCATCTTTTTCAGCCATC-3′ |
| IL-8 | 5′-TTGCCAAGGAGTGCTAAAGAA-3′ | 5′-GCCCTCTTCAAAAACTTCTCC-3′ |
| MCP-1 | 5′-AGGAACCGAGAGGCTGAGA-3′ | 5′-GGAATGAAGGTGGCTGCTAT-3′ |
| TGF-β | 5′-GCCAGAGTGGTTATCTTTTGATG-3′ | 5′-AGTGTGTTATCCCTGCTGTCAC-3′ |
| VEGF | 5′-AGGGCAGAATCATCACGAAGT-3′ | 5′-AGGGTCTCGATTGGATGGCA-3′ |
| GAPDH | 5′-CCACCCATGGCAAATTCCATGGCA-3′ | 5′-TCTACACGGCAGGTCAGGTCCACC-3′ |
| siRNA-CEACAM1 | 5′-CACCUUGAAUGUCACCUAUTT-3′ | 5′-AUAGGUGACAUUCAAGGUGTT-3′ |
| siRNA-NC | 5′-UUCUCCGAACGUGUCACGUTT-3′ | 5′-ACGUGACACGUUCGGAGAATT-3′ |
siRNA-NC is a negative control siRNA with the same nucleotide composition but which lacks significant sequence homology to CEACAM1