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. 2019 Apr 23;2019:8127301. doi: 10.1155/2019/8127301

Figure 4.

Figure 4

Effect of different sCD14 levels on the hPDLSC response to submaximal LTA concentration. Primary hPDLSCs were stimulated with LTA at submaximal concentration (10 ng/ml) and different concentrations of sCD14 (2.5-250 ng/ml). Untreated cells served as the control. After 24 hours of incubation, IL-6, CXCL8, and CCL2 gene expression levels (a) were measured using qPCR. The y-axis shows the n-fold expression of the target genes compared to the unstimulated control. The corresponding protein levels in conditioned media (b) were determined by ELISA. All data are presented as mean ± s.e.m. from five independent experiments using cells from five different donors. #p value < 0.05 compared to cells stimulated with LTA in the absence of sCD14.