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. 2019 Apr 4;8(8):e010823. doi: 10.1161/JAHA.118.010823

Figure 6.

Figure 6

Loss of Cx37 selectively alters the expression of the AT2 receptors in the aortas of Cx37−/− mice. A, Immunofluorescence showed a similar staining for AT1R (upper panels) in the aortas of all WT and Cx37−/− mice. In contrast, the staining for AT2R was more intense in the aortas of Cx37−/− than in WT mice. Bar=20 μm. L indicates lumen; M, media. B, Western blot showed that the aortas of WT and Cx37−/− mice expressed similar levels of the AT1R protein. C, In contrast, the basal levels of the AT2R protein were higher in Cx37−/− than in WT mice, and were not increased after the 2K1C surgery. WT sham N=21; Cx37−/− sham N=17; WT 2K1C N=19; Cx37−/− 2K1C N=17. D and E, Comparable observations were made for both AT1R (D) and AT2R (E) in mice infused daily with 1 mg Ang II/kg body weight WT NaCl N=4; Cx37−/− NaCl, Cx37−/− Ang II N=3, WT Ang II N=6. Results are means+SEM. ° P≤0.05 vs WT mice, as given by 2‐way ANOVA. 2K1C indicates 2‐kidney, 1‐clip; AngII, angiotensin II; AT1R, Ang II type 1 receptors; AT2R, Ang II type 2 receptors; Cx37, Connexin37; DAPI, 4′,6‐diamidino‐2‐phenylindole; Sham, sham‐operated controls; WT, wild type.