Table 1.
Protein A-tagged protein | Copies per diploid cell (asynchronous) |
---|---|
Cln1 | 995 ± 231 (4) |
Cln2 | 2011 ± 504 (5) |
Cln3 | 216 ± 44 (6) |
Clb1 | 497 ± 146 (4) |
Clb2 | 1128 ± 231 (4) |
Clb3 | 861 ± 231 (4) |
Clb4 | 445 ± 135 (4) |
Clb5 | 784 ± 193 (5) |
Clb6 | 92 ± 17 (4) |
Sic1 | 214 ± 42 (5) |
Cdc28 | 12,263 ± 3,130 (5) |
Extracts from log-phase diploid cultures (W303 background) using the NaOH/TCA method were analyzed for number of copies per cell of the indicated protein fused to protein A, as described in MATERIALS AND METHODS. Note that these are unsynchronized cultures, so the number of copies per cell measured is less than the peak number of copies per cell. Values are mean ± SEM, with the number of determinations in parentheses. Figure 4 presents a sample experiment in this series.