Table 1.

Quantitation of cyclins, Sic1, and Cdc28

Protein A-tagged protein	Copies per diploid cell (asynchronous)
Cln1	995  ± 231 (4)
Cln2	2011  ± 504 (5)
Cln3	216  ± 44 (6)
Clb1	497  ± 146 (4)
Clb2	1128  ± 231 (4)
Clb3	861  ± 231 (4)
Clb4	445  ± 135 (4)
Clb5	784  ± 193 (5)
Clb6	92  ± 17 (4)
Sic1	214  ± 42 (5)
Cdc28	12,263  ± 3,130 (5)

Extracts from log-phase diploid cultures (W303 background) using the NaOH/TCA method were analyzed for number of copies per cell of the indicated protein fused to protein A, as described in MATERIALS AND METHODS. Note that these are unsynchronized cultures, so the number of copies per cell measured is less than the peak number of copies per cell. Values are mean ± SEM, with the number of determinations in parentheses. Figure 4 presents a sample experiment in this series.