Fig. 2.

Number of transconjugants per plate when using different mating setups. a The same number of cells was subjected to different mating conditions. Conjugation was performed in liquid medium, applied as 1000 µl or 100 µl onto freshly poured plates (2 days) or as 1000 µl or 100 µl onto 2-week-old well-dried YTF agar plates (2 weeks). b Conjugation was performed using donor plasmids with the Clostridium origin of replication repL and repH. c E. coli strains with different dam and dcm methylation patterns were used as the donor strain containing pSOS-traJ repH. The data represent biological triplicates with standard deviations