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. 2019 May 8;20:231. doi: 10.1186/s12859-019-2865-7

Fig. 1.

Fig. 1

a SpliceV plot of SPPL2A expression in Akata cells. Coverage (exon level coverage; color intensity of each exon, single nucleotide level coverage; height of the horizontal line bisecting each exon) and forward splice junctions (arches above exons) was derived from sequencing a poly(A)-selected library preparation. Back-splice junctions (curves below exons) were obtained from sequencing a ribodepleted, RNase-R treated library preparation. b SpliceV plot of FARSA in Akata cells. All junctions and coverage were derived from a ribodepleted, RNase-R treated sample. c SpliceV plot of GSE1 expression in ribodepleted, RNase-R treated (back-splice junctions) and poly(A)-selected (coverage and canonical junctions) SNU719 cells. Predicted binding sites for RNA binding proteins, RBM3, HNRNPL, HNRNPA1, PTBP1 are plotted along the FARSA transcript (b) and RBFOX1, and MATR3 sites are plotted along the GSE1 transcript (c). ALU elements are marked in (c)