Table 1.
Oligonucleotide | Nucleotide sequence, 5′–3′ | Origin | Volume of reaction (nM) | Reference |
| ||||
Primers and probes for real-time PCR | ||||
| ||||
NDM-F Primer | GAC CGC CCA GAT CCT CAA | IDT | 300 | |
NDM-R Primer | CGC GAC CGG CAG GTT | IDT | 300 | |
NDM-probe | HEX-TG GAT CAA GCA GGA GAT-ZEN/IBFQ | IDT | 200 | |
KPC-F Primer | GGC CGC CGT GCA ATA C | IDT | 500 | |
KPC-R Primer | GCC GCC CAA CTC CTT CA | IDT | 500 | [32] |
KPC- probe | 6FAM-TG ATA ACG CCG CCG CCA ATT TGT-ZEN/IBFQ | IDT | 200 | |
16S rRNA-F Primer | TGG AGC ATG TGG TTT AAT TCG A | IDT | 200 | |
16S rRNA-R Primer | TGC GGG ACT TAA CCC AAC A | IDT | 200 | |
16S rRNA-probe | Cy5-CA CGA GCT GAC GAC AR∗C CAT GCA-IBRQ | IDT | 100 | |
| ||||
Primers for DNA sequencing | ||||
| ||||
NDM-F-seq | AGT CGC TTC CAA CGG TTT | IDT | 300 | This study |
NDM-R-seq | CAT TGG CAT AAG TCG CAA TCC | IDT | 300 | |
KPC-F-seq | GGT CAC CCA TCT CGG AAA | IDT | 500 | |
KPC-R-seq | GGG ATG GCG GAG TTC AG | IDT | 500 |