Skip to main content
. 2019 Apr 23;2019:9757625. doi: 10.1155/2019/9757625

Table 1.

Primers and probes used in this study.

Oligonucleotide Nucleotide sequence, 5′–3′ Origin Volume of reaction (nM) Reference

Primers and probes for real-time PCR

NDM-F Primer GAC CGC CCA GAT CCT CAA IDT 300
NDM-R Primer CGC GAC CGG CAG GTT IDT 300
NDM-probe HEX-TG GAT CAA GCA GGA GAT-ZEN/IBFQ IDT 200
KPC-F Primer GGC CGC CGT GCA ATA C IDT 500
KPC-R Primer GCC GCC CAA CTC CTT CA IDT 500 [32]
KPC- probe 6FAM-TG ATA ACG CCG CCG CCA ATT TGT-ZEN/IBFQ IDT 200
16S rRNA-F Primer TGG AGC ATG TGG TTT AAT TCG A IDT 200
16S rRNA-R Primer TGC GGG ACT TAA CCC AAC A IDT 200
16S rRNA-probe Cy5-CA CGA GCT GAC GAC ARC CAT GCA-IBRQ IDT 100

Primers for DNA sequencing

NDM-F-seq AGT CGC TTC CAA CGG TTT IDT 300 This study
NDM-R-seq CAT TGG CAT AAG TCG CAA TCC IDT 300
KPC-F-seq GGT CAC CCA TCT CGG AAA IDT 500
KPC-R-seq GGG ATG GCG GAG TTC AG IDT 500