Figure 1.

ARL2 and BART are expressed to the highest levels in neural tissues. The indicated rat tissue homogenates (25 μg of total protein/lane) were assayed by immunoblot for the presence of ARL2 and BART, as described under MATERIALS AND METHODS. Recombinant ARL2 (5 ng) or BART-His₆ (2 ng) was included as controls on the appropriate gel. This experiment was repeated multiple times with rat and mouse tissues and always gave similar results. Slight differences in mobility between controls and tissue lysates are discussed under MATERIALS AND METHODS.